What are the eIF5A interacting proteins?
What has been done?
Construction of the TIF51A-TAP strain
TAP Protocol
TAP method: Results
Profile of TAP-eIF5Aa Binding Proteins
The TIF51A-binding partner is 80S ribosome
TIF51A-ribosome interaction is sensitive to RNase and EDTA
Endogenous TIF51A co-sediments with ribosomes
80S ribosome is the interacting partner of eIF5A
Binding of eIF-5A to different populations of ribosomes
Binding of eIF-5Q to different populations of ribosomes
Is the eIF-5A-80S ribosome binding hypusine-dependent?
Stably transfected cells containing FLAG-tagged eIF5A
Hypusine-dependent eIF-5A-ribosome interaction in mammalian cells
Diagram 1
A temperature-sensitive mutant with eIF5A mutation
Effect of eIF5A on cytoskeleton and actin dynamics